microorganism carrying disruptions of multiple genes and a method of producing thereof
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United States of America
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Feb 25, 2025
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Dec 30, 2021
app pub date -
Nov 4, 2019
filing date -
Nov 4, 2019
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Abstract
The objective of the present invention is to provide a transformed Aspergillus microorganism lacking at least two types of selection marker genes available for marker recycling method and a composition therefor. The objective can be achieved by a transformed Aspergillus microorganism lacking at least two types of selection marker genes available for marker recycling method on its chromosomes, or a composition for transforming an Aspergillus microorganism containing at least two types of nucleic acid fragments containing a loop-out region and a selection marker gene available for marker recycling method between homologous recombination regions, wherein the selection marker genes contain a tryptophan biosynthesis gene and a gene different from tryptophan biosynthesis gene.
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