Automated system for high-throughput microinjection of adherent cells

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 12233406
APP PUB NO 20230059331A1
SERIAL NO

17393721

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

Two or more micropipettes are used to increase a microinjection throughput in an automated system for microinjecting adherent cells on a Petri dish. In the system, a motorized stage carrying the Petri dish sequentially visits the cells according to an optimized injection sequence. The sequence is selected by minimizing a total distance traveled by the motorized stage such that each cell is visited once by one of the micropipettes. Using multiple micropipettes advantageously reduces the minimized total distance over using a single micropipette to thereby increase the throughput. The optimized injection sequence is obtained by solving an equality-generalized traveling salesman problem. Each micropipette is mounted on a motorized micromanipulator. The motorized stage and motorized micromanipulators operate coordinately that each micromanipulator goes down or up during movement of the motorized stage to compensate for unevenness between a focus plane and a moving trajectory of the motorized stage.

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

  • CITY UNIVERSITY OF HONG KONG

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Shuxun Hong Kong, CN 4 2
Jiao, Yang Hong Kong, CN 51 223
Pan, Fei Hong Kong, CN 26 56
Sun, Dong Hong Kong, CN 38 211

Cited Art Landscape

Load Citation

Patent Citation Ranking

Forward Cite Landscape

Load Citation

Maintenance Fees

Fee Large entity fee small entity fee micro entity fee due date
3.5 Year Payment $1600.00 $800.00 $400.00 Aug 25, 2028
7.5 Year Payment $3600.00 $1800.00 $900.00 Aug 25, 2032
11.5 Year Payment $7400.00 $3700.00 $1850.00 Aug 25, 2036
Fee Large entity fee small entity fee micro entity fee
Surcharge - 3.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge - 7.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge - 11.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge after expiration - Late payment is unavoidable $700.00 $350.00 $175.00
Surcharge after expiration - Late payment is unintentional $1,640.00 $820.00 $410.00