Accelerated sequencing methods

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 12209278
APP PUB NO 20230060685A1
SERIAL NO

17822760

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Abstract

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Described herein are methods of generating a coupled sequencing read pair for a polynucleotide, and methods of analyzing the coupled sequencing read pair. The coupled sequencing read pair can be analyzed to detect polynucleotide variants, including at loci that are not directly sequenced within the coupled sequencing read pair. Other analytical methods can include using coupled sequencing read pairs to construct or validate a consensus sequence. The coupled sequencing read pair may be generated for a polynucleotide by generating sequencing data for a first region by extending a primer using labeled nucleotides; further extending the primer through a second region using nucleotides provided in a second region flow order, wherein primer extension through the second region is faster than primer extension through the first region; and generating sequencing data associated with a sequence of a third region of the polynucleotide by further extending the primer using labeled nucleotides.

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Patent Owner(s)

Patent OwnerAddress
ULTIMA GENOMICS INC7979 GATEWAY BLVD SUITE 101 NEWARK CA 94560

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Almogy, Gilad Palo Alto, US 206 4009
Barad, Omer Mazkeret Batya, IL 15 28
Brinza, Dumitru Montara, US 34 299
Etzioni, Yoav Tel Aviv, IL 18 40
Oberstrass, Florian Menlo Park, US 48 146
Pratt, Mark Bozeman, US 80 1545
Trepagnier, Eliane Oakland, US 10 23

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