N- and C-terminal tandem tag system for purification
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United States of America
Stats
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Jan 28, 2025
Grant Date -
Feb 24, 2022
app pub date -
Aug 20, 2021
filing date -
Aug 20, 2021
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
Expression vectors and methods of protein purification, which allow for selection of full length protein over truncated forms of the protein being purified, are disclosed. The methods express a target protein as a three domain fusion, represented by formula I:A-[Li]-B-[L2]-C, where A is a first purification tag domain, C is the second purification tag domain and B is the target protein domain. A, B and C are preferably covalently linked by linkers, L1 and L2 may be optional. The purification tags at the N and C termini are different. The purification tags at the N and C termini are different. Expression vectors including nucleic acid sequences which encode the fusion protein represented by formula I are also disclosed. The vectors are used with host expression systems such as insect, yeast, or mammalian cells to express the target protein, which is subsequently purified as a function of the different affinity tags.
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International Classification(s)
- 2021 Application Filing Year
- C12P Class
- 1408 Applications Filed
- 425 Patents Issued To-Date
- 30.19 % Issued To-Date
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- C12P Class
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