-mediated genetic transformation method for sea barleygrass

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United States of America Patent

PATENT NO 12203079
APP PUB NO 20230132082A1
SERIAL NO

17811764

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An Agrobacterium-mediated genetic transformation method for sea barleygrass is provided. The method includes: S1, selecting immature embryo materials of sea barleygrass with immature embryos each having a length in a range of 0.5-1.0, sterilizing them with alcohol and sodium hypochlorite to obtain sterilized seeds; S2, separating the immature embryos, crosscutting the immature embryos, and inducing callus generation and proliferation; S3, adjusting pre-culture time and Agrobacterium infection time of calli based on the callus generation and Agrobacterium growth to thereby prevent excessive Agrobacterium liquid; and S4, performing adventitious bud induction culture and rooting induction culture under a shielding-formed low-light environment to obtain tissue culture plantlets. It relates to a tissue culture method for immature embryos of sea barleygrass with high green spot differentiation and plantlet formation rates, which is not limited by materials. A transformation and regeneration system has high genetic transformation and mutation efficiency.

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Patent OwnerAddress
ZHEJIANG UNIVERSITY310058 YUHANG TANG ROAD XIHU DISTRICT HANGZHOU ZHEJIANG 866 HANGZHOU CITY ZHEJIANG PROVINCE 310058

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kuang, Liuhui Hangzhou, CN 1 0
Li, Qi Hangzhou, CN 318 2640
Wu, Dezhi Hangzhou, CN 1 0
Zhang, Guoping Hangzhou, CN 61 281

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