Mixture of branched secondary alcohol ethoxylates for removal of endotoxins in anion exchange chromatography

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United States of America Patent

PATENT NO 12203067
APP PUB NO 20230016026A1
SERIAL NO

17833675

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Abstract

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Provided herein is a method to reduce the endotoxin contamination in plasmid preparations. In the described method, plasmid DNA and endotoxins are bound to an anionic exchange matrix and are brought into contact with a wash buffer, comprising a combination of branched secondary alcohol ethoxylates with varying ethylene oxide chain lengths, wherein the branched secondary alcohol ethoxylates with the shorter ethoxylate chain is present in the washing buffer in excess compared to the branched secondary alcohol ethoxylate with the longer ethoxylate chain. The resulting purified plasmid has minimal endotoxin contamination levels and is considered endotoxin-free. Furthermore, provided are wash buffers comprising a combination of branched secondary alcohol ethoxylates with varying ethylene oxide chain lengths, kits comprising such wash buffers and the use of such wash buffers for reducing the endotoxin contamination in plasmid preparations.

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Patent Owner(s)

Patent OwnerAddress
AXAGARIUS GMBH & CO KG52355 DÜREN

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Heymans, Stefan Solingen, DE 3 2

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