Methods for preparing a sample for nucleic acid amplification using tagmentation
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United States of America
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Jan 7, 2025
Grant Date -
Jan 6, 2022
app pub date -
Sep 15, 2021
filing date -
Sep 15, 2021
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Abstract
Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of preparing nucleic acid containing cellular samples for library amplification, wherein the methods include lysing cells of the sample to form a lysate, amplifying the nucleic acids from the lysed samples, wherein there is no purification of the nucleic acids from the lysate prior to the amplification, wherein amplifying comprises tagmentation, and sequencing the nucleic acids, wherein the sample is a blood sample or a formalin-fixed paraffin-embedded (FFPE) sample.
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