Collection, genome editing, and washing of T-cell lymphocytes

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 12168087
APP PUB NO 20200282116A1
SERIAL NO

16807254

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Abstract

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Blood from a blood source is drawn into a fluid flow circuit. A mononuclear cell product is separated from the blood, followed by at least a portion of the mononuclear cell product being conveyed into an electroporation device without disconnecting the blood source from the fluid flow circuit. The electroporation device opens pores in a membrane of at least one of the cells of the mononuclear cell product to allow DNA material (which is added to the mononuclear cell product prior to electroporation) to enter and modify the genome of the cell. At least a portion of the modified mononuclear cell product is returned to the blood source. The mononuclear cell product may be washed prior to being conveyed into the electroporation device. The modified mononuclear cell product may be washed after exiting the electroporation device.

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Patent Owner(s)

Patent OwnerAddress
FENWAL INCTHREE CORPORATE DRIVE LAKE ZURICH IL 60047

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kusters, Benjamin E Pleasant Prairie, US 72 172
Min, Kyungyoon Kildeer, US 211 2278

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