Fluorescent polymerase enzyme substrates having protein shields

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 12162903
APP PUB NO 20230365614A1
SERIAL NO

18125072

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Abstract

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Compositions, methods, and systems are provided for fluorescent polymerase enzyme substrates comprising protein shields for improving enzyme photostability in single molecule real time sequencing. Fluorescent polymerase enzyme substrates of the invention have a protein shield between the fluorescent dye moieties and nucleotide moieties of the polymerase enzyme substrate. The polymerase enzyme substrates have a nucleotide component and a dye component, each attached to a protein. The attachments can be covalent. The protein can, for example, prevent the direct interaction of the fluorescent dye moiety with the enzyme when carrying out nucleotide synthesis, preventing photodamage to the enzyme. The polymerase enzyme substrates of the invention can have multiple dyes and multiple nucleotide moieties.

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Patent Owner(s)

Patent OwnerAddress
PACIFIC BIOSCIENCES OF CALIFORNIA INCMENLO PARK CA 94025

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bjornson, Keith Fremont, US 48 2799
Brogley, Louis Santa Cruz, US 31 589
Hanes, Jeremiah Woodside, US 54 1337
Kamtekar, Satwik Mountain View, US 50 708
Miller, Erik Berkeley, US 88 1047
Sebo, Lubomir Hayward, US 39 810

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