Cell culture clarification
Number of patents in Portfolio can not be more than 2000
United States of America
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Dec 3, 2024
Grant Date -
Feb 20, 2020
app pub date -
Mar 30, 2018
filing date -
Mar 30, 2018
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Abstract
A method and a kit for cell culture clarification, as well as a system or apparatus for clarifying a cell culture and purifying biomolecules of interest, are described. The cell culture comprises at least one biomolecule of interest. The method comprises adding one or more compounds to the cell culture that allow the formation of floccules of solutes and/or particulates in said cell culture harvest, adding an amount of diatomaceous earth (DE) to said cell culture harvest, agitating the obtained solution, and transferring said solution to a filtration vessel comprising a support filter having a surface, whereby the diatomaceous earth in said solution forms a layer on said surface and filtering said harvest solution through said layer and support filter.
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