Isolation and purification of anti-IL-13 antibodies using protein a affinity chromatography
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United States of America
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Dec 3, 2024
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Oct 27, 2022
app pub date -
May 17, 2022
filing date -
May 17, 2022
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Abstract
Disclosed herein are methods for the isolation and purification of anti-IL-13 antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography (e.g., Protein A affinity chromatography), ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
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