Biotin-streptavidin cleavage composition and library fragment cleavage

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United States of America

PATENT NO 12146133
APP PUB NO 20210238589A1
SERIAL NO

17166906

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Abstract

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An example of a biotin-streptavidin cleavage composition includes a formamide reagent and a salt buffer. The formamide reagent is present in the biotin-streptavidin cleavage composition in an amount ranging from about 10% to about 50%, based on a total volume of the biotin-streptavidin cleavage composition. The salt buffer makes up the balance of the biotin-streptavidin cleavage composition. In some examples, the biotin-streptavidin cleavage composition is used to cleave library fragments from a solid support. In other examples, other mechanisms are used to cleave library fragments from a solid support.

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Patent Owner(s)

  • ILLUMINA INC.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cao, Dan East Palo Alto, US 143 192
Fisher, Jeffrey S San Diego, US 57 757
Kaper, Fiona Solana Beach, US 34 1412
Khurana, Tarun Kumar Fremont, US 41 102
Liu, Tong Belmont, US 293 2685
Okumus, Burak San Diego, US 6 7
Quijano, Victor J Mountain View, US 1 0
Wang, Clifford Lee Berkeley, US 10 4
Wu, Yir-Shyuan Albany, US 59 624
Xiao, Shi Min Castro Valley, US 3 0
Xu, Hongxia Castro Valley, US 12 179

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