High-throughput crystallographic screening device and method for crystalizing membrane proteins using a sub physiological resting membrane potential across a lipid matrix of variable composition
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United States of America
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Oct 29, 2024
Grant Date -
Nov 16, 2023
app pub date -
Jul 25, 2023
filing date -
Jul 25, 2023
priority date (Note) -
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status (Latency Note)
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Abstract
The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under “cuasi” physiological conditions.
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International Classification(s)
- 2023 Application Filing Year
- B01L Class
- 1019 Applications Filed
- 187 Patents Issued To-Date
- 18.36 % Issued To-Date
Cited Art Landscape
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- 0 Citation Count
- B01L Class
- 0 % this patent is cited more than
- 1 Age
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