Aqueous two-phase system for the separation and recovery of mammalian cells from contaminated cultures
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United States of America Patent
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Oct 15, 2024
Grant Date -
Apr 30, 2020
app pub date -
Jul 3, 2018
filing date -
Jul 3, 2017
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Abstract
A method is directed to separating contaminants from mammalian cells in an aqueous multiphase system, and includes loading a container with a liquid having a top liquid phase and a bottom liquid phase. A cover medium is inserted in the container with a mixture of cultured mammalian cells and contaminants. The container is incubated and centrifuged for respective periods of time. In response to the centrifuging, the cells are separated from the contaminants in accordance with the respective density of the cells, the contaminants, and each liquid phase, resulting in the cells being located at a liquid-to-liquid interface between the top liquid phase and the bottom liquid phase and the contaminants being located at the bottom of the container.
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