Laser scanning microscope and method for determining a position of a fluorophore
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United States of America Patent
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Oct 8, 2024
Grant Date -
Jan 12, 2023
app pub date -
Nov 18, 2020
filing date -
Nov 18, 2020
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Abstract
A laser scanning microscope includes a light source configured to emit an illumination light beam. The illumination light beam has a transverse light intensity profile comprising an intensity minimum. The laser scanning microscope further includes a scanning device configured to scan the illumination light beam along a closed trajectory in a target area of a specimen, and a detector configured to detect fluorescence light emitted by a fluorophore within the target area of the specimen. The fluorophore is excited by the illumination light beam. The laser scanning microscope further includes a processor configured to determine an intensity distribution of the fluorescence light as a function of time and to determine a position of the fluorophore within the target area based on the intensity distribution of the fluorescence light.
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