and construction method and application thereof

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United States of America Patent

PATENT NO 12104191
APP PUB NO 20230084966A1
SERIAL NO

17823853

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Abstract

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A cofactor self-sufficient Escherichia coli and its construction method and application in the synthesis of L-glufosinate are provided. The present invention expresses a NADH kinase and key enzymes of the cofactor synthesis pathway in E. coli, and knocks out the genes of enzymes that catabolizes cofactor, and with the addition of co-metabolic intermediates during cell incubation, the intracellular NADP(H) concentration is increased by at least 50% and the catalytic activity of glufosinate dehydrogenase by 2-fold, resulting in a significant increase in the spatiotemporal yield of the glufosinate synthesis reaction.

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Patent Owner(s)

Patent OwnerAddress
ZHEJIANG UNIVERSITY OF TECHNOLOGY310014 HANGZHOU CITY IN THE LOWER REACHES OF THE CITY OF ZHEJIANG WANG ROAD NO 18 HANGZHOU CITY ZHEJIANG PROVINCE 310014

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cheng, Feng Zhejiang, CN 56 424
Jiang, Zhentao Zhejiang, CN 1 0
Xu, Jianmiao Zhejiang, CN 10 0
Xue, Yaping Zhejiang, CN 20 1
Zheng, Yuguo Zhejiang, CN 28 1
Zou, Shuping Zhejiang, CN 8 0

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