Cell-substrate impedance monitoring of cancer cells
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United States of America Patent
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Aug 20, 2024
Grant Date -
Feb 11, 2021
app pub date -
Oct 29, 2020
filing date -
Nov 20, 2015
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Abstract
Methods of assessing cytolysis of cancer cells are provided, which include: providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, adding target cells characterized as cancer cells to a well of the device adding effector cells to the well to form a test well, where the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance-based curves over time.
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