Method for producing L-theanine via fermentation by a genetically engineered bacterium and the application thereof

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United States of America Patent

PATENT NO 12065682
APP PUB NO 20230109256A1
SERIAL NO

17889397

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Abstract

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The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using an Escherichia coli genetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on an Escherichia coli W3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

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Patent Owner(s)

Patent OwnerAddress
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGYNO 9 13TH STREET BINHAI NEW AREA ECONOMIC AND TECHNOLOGICAL DEVELOPMENT ZONE TIANJIN TIANJIN CITY TIANJIN CITY 300000
HENAN JULONG BIOLOGICAL ENGINEERING CO LTDOVERPASS WEST GUANGCHENG ROAD RUZHOU HENAN 467500

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Ning Tianjin, CN 283 3274
Fan, Xiaoguang Tianjin, CN 7 6
Ji, Yuanqing Tianjin, CN 2 0
Li, Jing Henan, CN 1112 6098
Liu, Bochao Henan, CN 3 0
Liu, Shuai Henan, CN 116 166
Liu, Xiaodong Henan, CN 213 1292
Liu, Yongchao Henan, CN 45 42
Sun, Chaochao Henan, CN 12 19
Teng, Jiajia Henan, CN 2 0
Xu, Qingyang Tianjin, CN 10 5
Zhang, Mengtao Henan, CN 2 0
Zhou, Yuhang Tianjin, CN 9 4

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