Method for constructing trehalose multi-enzyme complex in vitro mediated by artificial scaffold protein

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PATENT NO 12043834
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17525283

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The present disclosure relates to a method for constructing a trehalose polyenzyme complex in vitro by mediation of an artificial scaffold protein, which mainly comprises the following steps: constructing a recombinant bacterium WB800n-ScafCCR for self-assembled scaffold protein module; constructing a recombinant bacterium WB800n-P43-phoD -treY-Ccdoc for self-assembled catalytic module; constructing a recombinant bacterium WB800n-P43-phoD-treZ-Ctdoc for self-assembled catalytic module; constructing a recombinant bacterium WB800n-P43-phoD-cgt-Rfdoc for self-assembled catalytic module; secretorily expressing the recombinant bacteria and self-assembling in vitro to obtain a recombinant trehalose multi-enzyme complex. The trehalose multi-enzyme complex constructed by the method of the present disclosure has a higher catalytic efficiency in preparing trehalose than that of mixed free enzymes, and the method can be used for production of high quality trehalose after immobilization with cellulose microspheres.

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QILU UNIVERSITY OF TECHNOLOGY250353 UNIVERSITY ROAD CHANGQING DISTRICT JI'NAN SHANDONG PROVINCE NO 3501 JINAN CITY SHANDONG PROVINCE 250353

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Liu, Hongling Jinan, CN 7 75
Wang, Ruiming Jinan, CN 9 7
Wang, Tengfei Jinan, CN 29 22
Zhang, Xinyi Jinan, CN 23 173

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