Rapid method for cloning and expression of cognate antibody variable gene segments
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United States of America
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Jul 2, 2024
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Jan 10, 2019
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Jan 10, 2019
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Abstract
In the method as reported herein the isolation of nucleic acid segments encoding antibody variable domains and the insertion of the isolated nucleic acid segments in eukaryotic expression plasmids is performed without the intermediate isolation and analysis of clonal intermediate plasmids. Thus, in the method as reported herein the intermediate cloning, isolation and analysis of intermediate plasmids is not required, e.g. by analysis of isolated transformed E. coli cells.
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