Method for producing psicose 3-epimerase by high-density fermentation

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United States of America Patent

PATENT NO 12012627
APP PUB NO 20230304057A1
SERIAL NO

18154827

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Abstract

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The present invention relates to the technical field of microbial fermentation engineering, and specifically to a method for producing psicose 3-epimerase by high-density fermentation. In view of the low expression level of psicose 3-epimerase and other problems existing in the current fermentation, by controlling the feeding rate in the fermentation process, improving the culture temperature in the middle and late stages of fermentation and other measures in the present invention, the OD value during the fermentation with recombinant Bacillus subtilis and the total enzyme activity of psicose 3-epimerase in the fermentation broth are significantly increased, the expression of psicose 3-epimerase is markedly improved, and the production cost of allulose is reduced. Therefore, the present invention has a very broad prospect of application in industry.

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Patent Owner(s)

Patent OwnerAddress
HENAN ZHONGDA HENGYUAN BIOTECHNOLOGY STOCK CO LTDEAST OF JINGER ROAD NORTH OF WEIER ROAD LINYING INDUSTRIAL PARK LUOHE CITY HENAN 462600

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Jin, Ziheng Luohe, CN 17 0
Li, Linzheng Luohe, CN 17 0
Wang, Sanying Luohe, CN 3 0
Wen, Yanjun Luohe, CN 14 0

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