Cellulosic enzyme recycling from separation of saccharified biomass
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United States of America Patent
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Jun 4, 2024
Grant Date -
Apr 6, 2023
app pub date -
Sep 30, 2022
filing date -
Mar 15, 2013
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Abstract
The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.
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