Mutant Uracil DNA glycosylase with improved thermal sensitivity
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United States of America Patent
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Jun 4, 2024
Grant Date -
Dec 15, 2022
app pub date -
Oct 22, 2020
filing date -
Oct 22, 2020
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Abstract
Provided is a mutant UDG having improved thermal sensitivity compared to a wild-type UDG. The mutant UDG of the presently claimed subject matter having a high thermal sensitivity has no inhibitory effect on the PCR reaction and thus can be advantageously used for the development of PCR/qPCR Premix and particularly PCR diagnostic kits employing UDG which requires the use of relatively low temperature in melting and amplification steps.
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