strain for producing succinic acid and construction method thereof

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United States of America Patent

PATENT NO 11976267
APP PUB NO 20220235314A1
SERIAL NO

17256906

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Abstract

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The invention provides a recombinant Escherichia coli strain for producing succinic acid and a construction method thereof. The by-product encoding genes in the E. coli strain FMME-N-2 are knocked out to obtain the E. coli strain FMME-N-5 (ΔfocA-pflB-ΔldhA-Δpta-ackA); and the phosphoenolpyruvate carboxykinase pck derived from Actinobacillus succinogenes and the phosphite dehydrogenase ptxD derived from Pseudomonas stutzeri were overexpressed. The constructed plasmid pTrcHisA-pck-ptxD was introduced into the expression host E. coli FMME-N-5 (ΔfocA-pflB-ΔldhA-Δpta-ackA), and the cells were screened in a plate containing ampicillin, to obtain an engineered strain E. coli FMME-N-5 (ΔfocA-pflB-ΔldhA-Δpta-ackA)-pck-ptxD that can efficiently produce succinic acid. After fermentation by a two-stage fermentation strategy, the production of succinic acid reaches 137 g/L, the yield of succinic acid is up to 1 g/g glucose, and the space time yield is 1.43 g/L/h, while no by-products of lactic acid and formic acid are accumulated, and the acetic acid content is 1-2 g/L.

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Patent Owner(s)

Patent OwnerAddress
JIANGNAN UNIVERSITYNO 1800 ROAD 214122 JIANGSU LIHU BINHU DISTRICT CITY OF WUXI PROVINCE WUXI CITY JIANGSU PROVINCE 214122

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Xiulai Wuxi, CN 9 2
Gao, Cong Wuxi, CN 21 5
Liu, Jia Wuxi, CN 387 1559
Liu, Liming Wuxi, CN 58 198
Luo, Qiuling Wuxi, CN 4 0
Shen, Chen Wuxi, CN 63 82
Song, Wei Wuxi, CN 301 954
Tang, Wenxiu Wuxi, CN 1 0

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