3D cell culture vessels for manual or automatic media exchange
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United States of America Patent
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Apr 30, 2024
Grant Date -
Aug 18, 2022
app pub date -
May 3, 2022
filing date -
Jul 14, 2017
priority date (Note) -
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Abstract
A cell culture vessel includes a base defining a base plane extending in a first direction and a second direction perpendicular to the first direction, a plurality of cell culture chambers stacked one atop another, each cell culture chamber having a top, a bottom and sidewalls, each of the top, bottom and sidewalls having an interior surface, wherein at least the bottom surface has an array of microcavities supporting the culture of cells as spheroids and each bottom surface is at an angle with respect to the plane of a table or surface upon which the vessel sits. Further, liquid can flow into each cell culture chamber via an inlet and out of each cell culture chamber via an outlet. The angled cell culture surface allows the cell culture chambers to be perfused or allows media changes without dislodging spheroids from microcavities.
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