Optimized microbial cells for production of melatonin and other compounds
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United States of America Patent
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Jan 9, 2024
Grant Date -
Mar 18, 2021
app pub date -
Oct 23, 2020
filing date -
Mar 31, 2016
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Abstract
Described herein are recombinant microbial host cells comprising biosynthetic pathways and their use in producing oxidation products and downstream products, e.g., melatonin and related compounds, as well as enzyme variants, nucleic acids, vectors and methods useful for preparing and using such cells. In specific aspects, the present invention relates to monooxygenases, e.g., amino acid hydroxylases, with a modified cofactor-dependency, and to enzyme variants and microbial cells providing for an improved supply of cofactors.
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