Compositions, methods, and systems for single cell barcoding and sequencing

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United States of America Patent

PATENT NO 11814619
APP PUB NO 20220389410A1
SERIAL NO

17834649

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Abstract

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This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), the plurality of droplets generated from an aqueous mixture comprising a set of single cells and a set of functionalized particles configured for a single cell assay. Upon generation, the plurality of droplets can be stabilized in position within a region of the collecting container, thereby providing a single-tube workflow for single cell analyses. Further, compositions implemented are structured to allow for overloading of partitions with functionalized particles, such that partitioned single-cells are co-localized with a subset of functionalized particles in a manner that allows for discernable tagging and downstream analyses.

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Patent Owner(s)

Patent OwnerAddress
COUNTABLE LABS INC1810 EMBARCADERO RD SUITE 200 PALO ALTO CA 94303

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Fan, Hei Mun Christina Palo Alto, US 24 71
Fodor, Stephen P A Palo Alto, US 155 27165
Lai, Janice Hoiyi Mountain View, US 15 62
Shum, Eleen Yee Lam San Carlos, US 19 71

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