Lithographic printing plate precursors are prepared with a unique aluminum-containing substrate and one or more radiation-sensitive imageable layers. The aluminum-containing substrate is prepared by three separate and sequential anodizing processes to provide an inner aluminum oxide layer having an average dry thickness (T_i) of 500-1,500 nm and a multiplicity of inner pores having an average inner pore diameter (D_i) larger than 0 and <15 nm. A formed middle aluminum oxide layer has a dry thickness (T_m) of 60-300 nm and a multiplicity of middle pores of average middle pore diameter (D_m) of 15-60 nm, arranged over the inner aluminum oxide layer. A formed outer aluminum oxide layer comprises a multiplicity of outer pores having an average outer pore diameter (D_o) of 5-35 nm and an average dry thickness (T_o) of 30-150 nm, arranged over the middle aluminum oxide layer. D_m is larger than D_o that is larger than D_i.

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