Genetically engineered strain with high yield of L-valine and method for producing L-valine by fermentation
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United States of America Patent
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Oct 31, 2023
Grant Date -
Apr 6, 2023
app pub date -
Oct 30, 2019
filing date -
Oct 24, 2019
priority date (Note) -
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status (Latency Note)
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Abstract
A genetically engineered strain having high-yield of L-valine is disclosed. Starting from Escherichia coli W3110, an acetolactate synthase gene alsS of Bacillus subtilis is inserted into a genome thereof and overexpressed; a ppGpp 3′-pyrophosphate hydrolase mutant R290E/K292D gene spoTM of Escherichia coli is inserted into the genome and overexpressed; a lactate dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB, and genes frdA, frdB, frdC, frdD of four subunits of fumaric acid reductase are deleted from the genome; a leucine dehydrogenase gene bcd of Bacillus subtilis replaces a branched chain amino acid transaminase gene ilvE of Escherichia coli; and an acetohydroxy acid isomeroreductase mutant L67E/R68F/K75E gene ilvCM replaces the native acetohydroxy acid isomeroreductase gene ilvC of Escherichia coli. Furthermore, the L-valine fermentation method is improved by using a two-stage dissolved oxygen control. The L-valine titer and the sugar-acid conversion rate are increased.
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International Classification(s)
- 2019 Application Filing Year
- C12R Class
- 167 Applications Filed
- 65 Patents Issued To-Date
- 38.93 % Issued To-Date
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- C12R Class
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