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United States of America Patent

PATENT NO 11800842
APP PUB NO 20230180689A1
SERIAL NO

18079850

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Abstract

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The present disclosure provides a method for obtaining adventitious tetraploid buds of Blumea balsamifera, comprising the following steps: selecting a root segment of diploid B. balsamifera as an explant, culturing the root segment in a chromosome doubling inducing medium supplemented with 0.025-0.1 mg/L 1-naphthaleneacetic acid (NAA), 1.0-2.0 mg/L 6-benzylaminopurine (6-BA), and 90-150 mg/L colchicine, inducing explant cells, and simultaneously doubling chromosomes and differentiating the adventitious buds. The present disclosure fills the blank of using a root of B. balsamifera as the explant and increases effective explant sources during the propagation, proliferation and biotechnological breeding of B. balsamifera. More importantly, root cells of the B. balsamifera are directly differentiated into adventitious buds while chromosomes are doubled, and a callus formation process is not needed, so that the technical links are simplified and the variation of regeneration buds and the generation of chimeras are reduced.

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Patent Owner(s)

Patent OwnerAddress
TROPICAL CROPS GENETIC RESOURCES INSTITUTE CHINESE ACADEMY OF TROPICAL AGRICULTURAL SCIENCESNO 4 XUEYUAN ROAD LONGHUA DISTRICT HAIKOU HAINAN 571101

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Xiaolu Haikou, CN 22 153
Chen, Yinghua Haikou, CN 4 26
Chen, Zhenxia Haikou, CN 14 16
Guan, Lingliang Haikou, CN 1 0
Huang, Mei Haikou, CN 30 122
Li, Yulan Haikou, CN 51 188
Luo, Qin Haikou, CN 18 55
Wang, Dan Haikou, CN 314 948
Wang, Kai Haikou, CN 565 3689
Xiao, Yongfeng Haikou, CN 2 0
Xie, Xiaoli Haikou, CN 6 2
Yang, Yong Haikou, CN 636 3335
Yu, Fulai Haikou, CN 1 0
Yu, Ping Haikou, CN 167 874
Yuan, Chao Haikou, CN 126 2678
Zhang, Yingbo Haikou, CN 16 40

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