Method for multiplying nucleic acids

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United States of America Patent

PATENT NO 11795502
APP PUB NO 20200109439A1
SERIAL NO

16599754

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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One or more nanoparticles, each of which is conjugated with at least one oligonucleotide, are used to multiply nucleic acids. One or more of the oligonucleotides has at least one primer sequence and an additional segment extending from the end of the primer sequence proximal to the nanoparticle in the direction of the nanoparticle, and the additional segment has at least one abasic modification. The disclosed method for multiplying nucleic acids has a multiplication step and a test step for determining the concentration of the products of the multiplication reaction. The test step begins after the multiplication step ends, and in the test step, either at least one part of the sample is supplied with substances or no substances are supplied. In a method for multiplying nucleic acids, nanoparticles transfer heat into their surroundings in a reaction volume upon being excited. A method for multiplying a nucleic acid has an amplification step that uses a polymerase chain reaction, where a cycle consisting of the steps of denaturing, annealing, and elongating is repeated.

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Patent Owner(s)

Patent OwnerAddress
HP HEALTH SOLUTIONS GERMANY GMBHAM KLOPFERSPITZ 19 MARTINSRIED D-82152

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Buersgens, Federico Planegg/Martinsried, DE 10 12
Rebuffo-Scheer, Cecilia Planegg-Martinsried, DE 6 5
Stehr, Joachim Planegg/Martinsried, DE 15 17
Ullerich, Lars Planegg/Martinsried, DE 15 17

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