Method for enzymatic preparation of glutathione

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United States of America Patent

PATENT NO 11788110
SERIAL NO

16622856

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Abstract

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The present invention relates an enzymatic method for preparing glutathione, comprising the following steps: (1) producing glutathione in a reactor using GshF enzyme, ATP regeneration enzyme and AK enzyme; (2) separating immobilized GshF enzyme, ATP regeneration enzyme and AK enzyme in the reactor, or separating free GshF enzyme using a filtration device; (3) separating the filtrate obtained in step (2) to obtain a crude product GSH and a small amount of ATP, ADP and AMP; and (4) subjecting the GSH obtained in step (3) to concentration, crystallization, and drying to prepare a finished GSH. The described method provides: greatly reduced industrial production costs; faster reaction rate; stable enzyme recovery system that is energy efficient and environmentally friendly; and capability of reusing the byproducts or collecting them for the production of ATP.

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Patent Owner(s)

Patent OwnerAddress
ANHUI GSH BIO-TECH CO LTDFENGHUANG BUILDING 4-1-1003 HUANCHENGXI ROAD 99 DAGUAN DISTRICT ANQING ANHUI

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Liu, Hui Beijing, CN 520 7207
Liu, Shanshan Beijing, CN 51 70
Qin, Yongfa Beijing, CN 4 2
Zhou, Wenwen Beijing, CN 8 4

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