Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Oct 10, 2023
Grant Date -
Aug 19, 2021
app pub date -
May 13, 2019
filing date -
May 24, 2018
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
The present invention provides mammalian cell expression vectors that impart to mammalian host cells an ability to produce high levels of foreign gene-derived proteins. A ubiquitously acting chromatin opening element (UCOE) is introduced into an expression vector that has a plasmid DNA integrated into a transcriptional hot spot on the chromosome of a dihydrofolate reductase gene-deficient host cell so that it allows for selection of strains that grow in hypoxanthine-thymidine (hereinafter denoted as HT)-free medium, whereby transformants will produce a protein of interest in increased amounts.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully