for synthesizing lacto-N-neotetraose and application thereof

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United States of America Patent

PATENT NO 11761021
APP PUB NO 20200140894A1
SERIAL NO

16747016

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The disclosure discloses recombinant Bacillus subtilis for synthesizing e lacto-N-neotetraose yield. The recombinant Bacillus subtilis is obtained by integrating two β-1,4-galactotransferase genes on a genome of a host bacterium Bacillus subtilis 168ΔamyE:P43-lacY, P43-lgtB, PxylA-comK and exogenously expressing a β-1,3-N-glucosaminotransferase gene. Compared with a strain before transformation, the recombinant Bacillus subtilis of the disclosure improves the yield of the synthesized lacto-N-neotetraose from 720 mg/L to 1300 mg/L, laying a foundation for further metabolic engineering transformation of Bacillus subtilis for producing the lacto-N-neotetraose.

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Patent Owner(s)

Patent OwnerAddress
JIANGNAN UNIVERSITYNO 1800 ROAD 214122 JIANGSU LIHU BINHU DISTRICT CITY OF WUXI PROVINCE WUXI CITY JIANGSU PROVINCE 214122
BRIGHT DAIRY & FOOD CO LTD201103 NO 578 MINHANG DISTRICT SHANGHAI WUZHONG ROAD SHANGHAI CITY SHANGHAI CITY 201103

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Jian Wuxi, CN 1588 23569
Dong, Xiaomin Wuxi, CN 3 1
Du, Guocheng Wuxi, CN 67 10
Li, Jianghua Wuxi, CN 39 53
Liu, Long Wuxi, CN 70 43
Lv, Xueqin Wuxi, CN 14 2
Wang, Miao Wuxi, CN 160 792

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