Compositions and methods for high efficiency protein precipitation
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United States of America Patent
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Sep 19, 2023
Grant Date -
Mar 25, 2021
app pub date -
Sep 23, 2020
filing date -
Sep 20, 2019
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Abstract
Methods for isolating proteins from solution by precipitation and compositions generated thereby are provided. A nonvolatile precipitation agent is added to an aqueous protein solution at a low concentration. Water is then removed from the resulting solution until the precipitant and the protein content of the solution increase to a concentration that provides the desired segregation of proteins between supernatant and precipitate. Additional water can be removed from the supernatant to provide additional fractionation. Water can be removed by evaporation (e.g. under reduced pressure) and/or diafiltration.
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