Fermentation methods for producing steviol glycosides with multi-phase feeding
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United States of America Patent
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Aug 29, 2023
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app pub date -
Dec 7, 2021
filing date -
May 29, 2015
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Abstract
Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. The methods include at least two phases: first and second phases where a glucose-containing feed composition is provided to the medium in different modes of feeding in each phase, such as variable feeding and then constant feeding. The two phase feeding can result in a growth rate that is slower in the second phase than in the first phase, and consequently increased steviol glycoside production rates, reduced fermentation times, and reduced biomass concentrations.
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