Rapid three-dimensional imaging system and method based on multi-angle 4Pi microscope, and storage medium
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United States of America Patent
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Aug 8, 2023
Grant Date -
Dec 17, 2020
app pub date -
May 7, 2020
filing date -
Jun 11, 2019
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Abstract
The present disclosure provides a rapid three-dimensional imaging system based on a multi-angle 4Pi microscope. The system includes: an illumination module, configured to obtain a parallel light of which a size covering a projection surface of a spatial light modulator; a wavefront modulation module, configured to place the LCOS device on a Fourier plane of an illumination end; a two-dimensional scanning module, configured to control a light beam to realize a two-dimensional scanning on an object plane; an illumination interference module, configured to generate point spread function PSFs of a 4Pi through an illumination interference to irradiate a fluorescent sample; an imaging module, configured to acquire interference images of two fluorescent signals; and a controller, configured to control the wavefront modulation module to adjust a polarization direction of the light to generate PSFs of the 4Pi with different inclination angles.
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