System for rapid, portable, and multiplexed detection and identification of pathogen specific nucleic acid sequences

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United States of America Patent

PATENT NO 11654429
SERIAL NO

16495614

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Abstract

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A sample carrier may include a sample preparation module and an amplification module. A sample mixes with a lysis medium and a nucleic acid amplification medium in the sample preparation module and then flows into a plurality of microfluidic chambers in the amplification module. The microfluidic chambers have disposed therein primers configured to initiate amplification of one or more target nucleic acid sequences corresponding to one or more pathogens. The sample carrier is inserted into an apparatus that includes a plurality of Sight sources and a camera. The light sources illuminate the microfluidic chambers with excitation light, a fluorophore emits fluorescence light indicative of nucleic acid amplification in response to the excitation-light, and the camera captures images of the microfluidic chambers. A target nucleic acid sequence in the sample is indicated by the images showing an increasing fluorescence in a microfluidic chamber that has the primers for that sequence.

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Patent Owner(s)

Patent OwnerAddress
THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS352 HENRY ADMINISTRATION BUILDING 506 S WRIGHT ST URBANA IL 61801

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bashir, Rashid Champaign, US 79 2503
Chen, Weili Sunnyvale, US 13 89
Cunningham, Brian T Champaign, US 91 2682
Damhorst, Gregory Elgin, US 2 10
Ganguli, Anurup Urbana, US 38 45
Ornob, Akid Champaign, US 1 2
Sun, Fu Urbana, US 4 3
Yu, Hojeong Savoy, US 2 2

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