Bead incubation and washing on a droplet actuator

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United States of America Patent

PATENT NO 11525827
APP PUB NO 20200209231A1
SERIAL NO

16811789

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Abstract

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Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.

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Patent Owner(s)

Patent OwnerAddress
ADVANCED LIQUID LOGIC INC5200 ILLUMINA WAY SAN DIEGO CA 92122

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Pamula, Vamsee K Cary, US 132 12386
Pollack, Michael G Durham, US 139 12816
Sista, Ramakrishna Cary, US 45 1544
Sudarsan, Arjun Carlsbad, US 44 1661

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