Microfluidic system for sperm separation and enrichment from various types of sperm samples
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United States of America Patent
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Dec 6, 2022
Grant Date -
Oct 31, 2019
app pub date -
Oct 29, 2018
filing date -
Oct 27, 2017
priority date (Note) -
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Abstract
A method for separating and enriching sperm from a tissue sample comprises: obtaining a microfluidic separating system having an inlet end and an outlet end, and a membrane filter (e.g., hollow fiber membrane filter) fluidly connected to the outlet end; separating the tissue sample via the microfluidic separating system into a debris fluid volume and a sperm fluid volume; and enriching the sperm fluid volume by removing excess media via the membrane filter. A two-stage tissue sample separation system comprising: a microchannel structure defining a separation fluid channel to form a separation stage; an inlet end of the microchannel structure; an outlet end of the microchannel structure; and a membrane filter fluidly connected to the outlet end for removal of at least a portion of excess media in the tissue sample.
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