Polypeptides having pullulanase activity suitable for use in liquefaction

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United States of America Patent

PATENT NO 11427811
APP PUB NO 20180208917A1
SERIAL NO

15746162

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Abstract

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The present invention relates to a variant pullulanase, wherein the pullulanase comprises at least the following combination of substitutions: N368G+N393A+Q431E+L432F+A492A,S+N610R+G624S+T631S+S632C, and optionally further comprises N222P+Q252A+Q256R; wherein the variant has pullulanase activity, and wherein the variants have at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3. Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.

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Patent Owner(s)

Patent OwnerAddress
NOVOZYMES A/SDENMARK BAGSVAERD BAGSVAERD REGION HOVEDSTADEN

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Clark, Suzanne Youngsville, US 30 125
Matsui, Tomoko Chiba, JP 72 408
Tomiki, Aki Chiba, JP 8 8

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