Particle-based immunoassay using a pegylated analyte-specific binding agent

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United States of America Patent

PATENT NO 11391733
APP PUB NO 20180172680A1
SERIAL NO

15899003

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Abstract

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Disclosed is a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein the microparticles are coated with the first partner of a binding pair, the method involving mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of containing or containing the analyte, wherein the second partner of the binding pair is bound to the analyte-specific binding agent via a linker having from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.

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Patent Owner(s)

Patent OwnerAddress
ROCHE DIAGNOSTICS OPERATIONS INC9115 HAGUE ROAD INDIANAPOLIS IN 46250

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Heindl, Dieter Penzberg, DE 143 1272
Nichtl, Alfons Penzberg, DE 12 81
Sicherl-Birk, Frank Penzberg, DE 2 0
Zarnt, Toralf Penzberg, DE 17 15

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