Proteinases with improved properties
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United States of America Patent
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Jul 19, 2022
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N/A
app pub date -
Dec 21, 2020
filing date -
Dec 20, 2018
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Abstract
Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.
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