Fully integrated hand-held device to detect specific nucleic acid sequences
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United States of America
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Jun 28, 2022
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Feb 7, 2019
app pub date -
Jan 9, 2017
filing date -
Jan 9, 2017
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Abstract
A fully integrated and disposable point-of-care device for detecting a target nucleic acid is provided. The device comprises: an extraction chamber adapted to receive a biological sample, wherein said extraction chamber comprises means to extract and lyse the sample to release nucleic acid; a first amplification chamber in communication with the extraction chamber, wherein said amplification chamber comprises means to trigger nucleic acid amplification of a target nucleic acid sequence to occur; and a detection chamber in communication with the amplification chamber, wherein said detection chamber comprises means to detectably label the target nucleic acid and means to detect a signal associated with labeled target nucleic acid, or a single chamber for amplification, detection and identification of multiple nucleic acid sequences.
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