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United States of America Patent
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May 24, 2022
Grant Date -
Oct 31, 2019
app pub date -
Dec 26, 2017
filing date -
Jan 1, 2017
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Abstract
Direct detection of mutagenesis in prokaryotes by reversion of an inactivating mutation (reversion mutation assay), producing a quantitative signal for in vivo mutagenesis, may greatly reduce the amount of test chemicals and labor involved in these assays. Further, transcriptional coupling of β-lactamase reversion and GFP, translational fusion between β-lactamase and GFP with stop codon in GFP, and a novel dual reporter to monitor continuous mutagenesis may be used in methods described herein.
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