Buoyancy-activated cell sorting (BACS)-compatible activation/transduction systems and methods
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United States of America Patent
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Oct 12, 2021
Grant Date -
Sep 19, 2019
app pub date -
Mar 14, 2019
filing date -
Mar 14, 2018
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Abstract
Disclosed herein are methods for contacting in a closed container a host liquid including target cells, microbubble reagents comprising gas-core lipid-shelled microbubbles, and one or more antibodies or other ligands that bind to cell surface molecules on the target cells, wherein the one or more antibodies or other ligands are bound to the target cells or the microbubbles, wherein the contacting under conditions to produce target cells linked to microbubbles via the one or more antibodies or other ligands and activating the target cells to generate activated target cells.
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