Optogenetic tool for rapid and reversible clustering of proteins

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 11053491
APP PUB NO 20200109392A1
SERIAL NO

16701399

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Abstract

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A protein construct including a gene encoding a light-sensitive protein fused to at least one of either a low complexity sequence, an intrinsically disordered protein region (IDR), or a repeating sequence of a linker and another gene encoding a light-sensitive protein. Among the many different possibilities contemplated, the protein construct may also advantageously include cleavage tags. This protein construct may be utilized for a variety of functions, including a method for protein purification, which requires introducing the protein construct into a living cell, and inducing the formation of clusters by irradiating the construct with light. The method may also advantageously include cleaving a target protein from an IDR, and separating the clusters via centrifuge. A kit for practicing in vivo aggregation or liquid-liquid phase separation is also included, the kit including the protein construct and a light source capable of producing a wavelength that the light-sensitive protein will respond to.

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Patent Owner(s)

Patent OwnerAddress
THE TRUSTEES OF PRINCETON UNIVERSITYPRINCETON UNIVERSITY OFFICE OF TECHNOLOGY LICENSING PRINCETON NJ 08544

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Brangwynne, Cliff Hopewell, US 8 11
Shin, Yongdae Lawrenceville, US 4 6
Toettcher, Jared Princeton, US 4 5

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