Methods of generating bioactive peptide-bearing antibodies and compositions comprising the same
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United States of America Patent
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Jun 29, 2021
Grant Date -
Aug 31, 2017
app pub date -
Mar 15, 2017
filing date -
Mar 15, 2013
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Abstract
In one aspect, the invention provides a method of making a bioactive peptide-bearing antibody, or fragment thereof, comprising (a) engrafting the amino acid sequence of at least one bioactive peptide of interest into (i) at least one of CDR-H1, CDR-H2 or CDR-H3 of a heavy chain variable region comprising one or more chicken framework regions and/or (ii) at least one of CDR-L1, CDR-L2 or CDR-L3 of the light chain variable region comprising one or more chicken framework regions, and (b) determining whether the antibody has substantially the same biological activity as the bioactive peptide.
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