Culture device for lactic acid bacteria
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United States of America Patent
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May 4, 2021
Grant Date -
Nov 1, 2018
app pub date -
Apr 26, 2016
filing date -
Apr 29, 2015
priority date (Note) -
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status (Latency Note)
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Abstract
The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.
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