Method for concentrating a cell suspension comprising a mucilaginous biomass of oleaginous yeasts
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United States of America Patent
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Mar 30, 2021
Grant Date -
Aug 9, 2018
app pub date -
Aug 5, 2016
filing date -
Aug 6, 2015
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Abstract
A method for concentrating, in order to favour the subsequent extraction process of intracellular lipids, a cell suspension containing a biomass of oleaginous yeasts fermented in fermentation broth under conditions that allow the intracellular accumulation of lipids, where the biomass contains significant quantities of mucilaginous material. The method includes:
- a) cultivating the oleaginous yeasts in a fermentation broth to obtain a cell suspension containing the mucilaginous biomass;b) subjecting the cell suspension obtained from a) to heat treatment, at a temperature between 95° C. and 120° C. and to acid treatment, to obtain a treated cell suspension containing the mucilaginous biomass containing intact oleaginous yeast cells; andc) concentrating the treated cell suspension obtained from b), by removing at least part of the fermentation broth to obtain a concentrated cell suspension.
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